Experts in the field, including Oncode Investigators Bas van Steensel and Elzo de Wit (both NKI), Jop Kind and Wouter de Laat (both Hubrecht Institute), and Ruud Delwel (ErasmusMC), discussed how the 3D genome influences developmental processes and can affect disease, and their experience with genome-wide methods such as (single cell) DamID and Hi-C. The speakers also shared tips and tricks for data analysis. The masterclass participants appreciated the wide range of techniques discussed, including the historical background, recent findings and technique optimizations that were mentioned.

During the afternoon practical session, the participants were given the chance to analyze Hi-C datasets on their own with the Hi-C analysis software package GENOVA, which was developed by the instructors, Robin van der Weide, Elzo de Wit and Teun van der Brand (all NKI), who also provided a detailed explanation of the tools available in the GENOVA package.

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We were pleased to see the involvement of the audience, who contributed with their involvement and high-quality questions, and a good representation of female scientists. The feedback that we received for this masterclass was very positive and enthusiastic, and we’d like to thank everyone involved for helping us deliver a successful masterclass.

Next in the series of our masterclasses and workshops is the Advanced workshop: Preclinical tumor immune models for therapeutic discovery and validation, organized by Linde Meyaard (UMCU) and Daniel Peeper (NKI), which will be held on July 1 at the NKI in Amsterdam.

If you have any questions or would like to get involved, please contact Colette ten Hove.